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Maleimide scavenging enhances determination of protein S-palmitoylation state in acyl-exchange methods
S-palmitoylation (S-acylation) is an emerging dynamic post-translational modification of cysteine residues within proteins. Current assays for protein S-palmitoylation involve either in vivo labelling or chemical cleavage of S-palmitoyl groups to reveal a free cysteine sulfhydryl that can be subsequ...
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| Veröffentlicht in: | Biotechniques |
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| Hauptverfasser: | , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2017
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5400063/ https://ncbi.nlm.nih.gov/pubmed/28193150 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000114516 |
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