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Mini-G proteins: Novel tools for studying GPCRs in their active conformation

Mini-G proteins are the engineered GTPase domains of Gα subunits. They couple to GPCRs and recapitulate the increase in agonist affinity observed upon coupling of a native heterotrimeric G protein. Given the small size and stability of mini-G proteins, and their ease of expression and purification,...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Nehmé, Rony, Carpenter, Byron, Singhal, Ankita, Strege, Annette, Edwards, Patricia C., White, Courtney F., Du, Haijuan, Grisshammer, Reinhard, Tate, Christopher G.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5398546/
https://ncbi.nlm.nih.gov/pubmed/28426733
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0175642
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