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Multicolour nanoscopy of fixed and living cells with a single STED beam and hyperspectral detection

The extension of fluorescence nanoscopy to larger numbers of molecular species concurrently visualized by distinct markers is of great importance for advanced biological applications. To date, up to four markers had been distinguished in STED experiments featuring comparatively elaborate imaging sch...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Winter, Franziska R., Loidolt, Maria, Westphal, Volker, Butkevich, Alexey N., Gregor, Carola, Sahl, Steffen J., Hell, Stefan W.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5394456/
https://ncbi.nlm.nih.gov/pubmed/28417977
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep46492
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