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Novel reversibly switchable fluorescent proteins for RESOLFT and STED nanoscopy engineered from the bacterial photoreceptor YtvA

The reversibly switchable fluorescent proteins (RSFPs) commonly used for RESOLFT nanoscopy have been developed from fluorescent proteins of the GFP superfamily. These proteins are bright, but exhibit several drawbacks such as relatively large size, oxygen-dependence, sensitivity to low pH, and limit...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Gregor, Carola, Sidenstein, Sven C., Andresen, Martin, Sahl, Steffen J., Danzl, Johann G., Hell, Stefan W.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5807511/
https://ncbi.nlm.nih.gov/pubmed/29426833
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-19947-1
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