A new sensitive LC/MS/MS analysis of vitamin D metabolites using a click derivatization reagent, 2-nitrosopyridine

There is an increased demand for comprehensive analysis of vitamin D metabolites. This is a major challenge, especially for 1α,25-dihydroxyvitamin D [1α,25(OH)(2)VitD], because it is biologically active at picomolar concentrations. 4-Phenyl-1,2,4-triazoline-3,5-dione (PTAD) was a revolutionary reage...

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Detalhes bibliográficos
Publicado no:J Lipid Res
Main Authors: Wan, Debin, Yang, Jun, Barnych, Bogdan, Hwang, Sung Hee, Lee, Kin Sing Stephen, Cui, Yongliang, Niu, Jun, Watsky, Mitchell A., Hammock, Bruce D.
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Biochemistry and Molecular Biology 2017
Assuntos:
Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5392736/
https://ncbi.nlm.nih.gov/pubmed/28148721
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.D073536
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