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Selenocysteine Insertion at a Predefined UAG Codon in a Release Factor 1 (RF1)-depleted Escherichia coli Host Strain Bypasses Species Barriers in Recombinant Selenoprotein Translation

Selenoproteins contain the amino acid selenocysteine (Sec), co-translationally inserted at a predefined UGA opal codon by means of Sec-specific translation machineries. In Escherichia coli, this process is dependent upon binding of the Sec-dedicated elongation factor SelB to a Sec insertion sequence...

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Detalhes bibliográficos
Publicado no:J Biol Chem
Main Authors: Cheng, Qing, Arnér, Elias S. J.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5392690/
https://ncbi.nlm.nih.gov/pubmed/28193838
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M117.776310
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