Optimized solubilization of TRIzol-precipitated protein permits Western blotting analysis to maximize data available from brain tissue

BACKGROUND: Techniques simultaneously assessing multiple levels of molecular processing are appealing because molecular signaling underlying complex neural phenomena occurs at complementary levels. The TRIzol method isolates RNA and DNA, but protein retrieval is difficult due to inefficient solubili...

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Udgivet i:J Neurosci Methods
Main Authors: Kopec, Ashley M., Rivera, Phillip D., Lacagnina, Michael J., Hanamsagar, Richa, Bilbo, Staci D.
Format: Artigo
Sprog:Inglês
Udgivet: 2017
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5392113/
https://ncbi.nlm.nih.gov/pubmed/28192129
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jneumeth.2017.02.002
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