Optimized solubilization of TRIzol-precipitated protein permits Western blotting analysis to maximize data available from brain tissue

BACKGROUND: Techniques simultaneously assessing multiple levels of molecular processing are appealing because molecular signaling underlying complex neural phenomena occurs at complementary levels. The TRIzol method isolates RNA and DNA, but protein retrieval is difficult due to inefficient solubili...

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Detalles Bibliográficos
Publicado en:J Neurosci Methods
Autores principales: Kopec, Ashley M., Rivera, Phillip D., Lacagnina, Michael J., Hanamsagar, Richa, Bilbo, Staci D.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2017
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Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC5392113/
https://ncbi.nlm.nih.gov/pubmed/28192129
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jneumeth.2017.02.002
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