QuickLib, a method for building fully synthetic plasmid libraries by seamless cloning of degenerate oligonucleotides

Incorporation of synthetic degenerate oligonucleotides into plasmids for building highly diverse genetic libraries requires efficient and quantitative DNA manipulation. We present a fast and seamless method for generating libraries of PCR-synthesized plasmids designed with a degenerate sequence and...

Descripció completa

Guardat en:
Dades bibliogràfiques
Publicat a:PLoS One
Autors principals: Galka, Pierre, Jamez, Elisabeth, Joachim, Gilles, Soumillion, Patrice
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2017
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5390991/
https://ncbi.nlm.nih.gov/pubmed/28406948
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0175146
Etiquetes: Afegir etiqueta
Sense etiquetes, Sigues el primer a etiquetar aquest registre!

Ítems similars