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QuickLib, a method for building fully synthetic plasmid libraries by seamless cloning of degenerate oligonucleotides
Incorporation of synthetic degenerate oligonucleotides into plasmids for building highly diverse genetic libraries requires efficient and quantitative DNA manipulation. We present a fast and seamless method for generating libraries of PCR-synthesized plasmids designed with a degenerate sequence and...
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| Veröffentlicht in: | PLoS One |
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| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Public Library of Science
2017
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5390991/ https://ncbi.nlm.nih.gov/pubmed/28406948 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0175146 |
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