A Fast and Effective Microfluidic Spraying-plunging Method for High-Resolution Single-Particle Cryo-EM

We describe a spraying-plunging method for preparing cryo-EM grids with vitreous ice of controllable, highly consistent thickness using a microfluidic device. The new polydimethylsiloxane (PDMS)-based sprayer was tested with apoferritin. We demonstrate that the structure can be solved to high resolu...

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Bibliografiske detaljer
Udgivet i:Structure
Main Authors: Feng, Xiangsong, Fu, Ziao, Kaledhonkar, Sandip, Jia, Yuan, Shah, Binita, Jin, Amy, Liu, Zheng, Sun, Ming, Chen, Bo, Grassucci, Robert A., Ren, Yukun, Jiang, Hongyuan, Frank, Joachim, Lin, Qiao
Format: Artigo
Sprog:Inglês
Udgivet: 2017
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5382802/
https://ncbi.nlm.nih.gov/pubmed/28286002
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.str.2017.02.005
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