A robust protocol for efficient generation, and genomic characterization of insertional mutants of Chlamydomonas reinhardtii

BACKGROUND: Random insertional mutagenesis of Chlamydomonas reinhardtii using drug resistance cassettes has contributed to the generation of tens of thousands of transformants in dozens of labs around the world. In many instances these insertional mutants have helped elucidate the genetic basis of v...

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Detalhes bibliográficos
Publicado no:Plant Methods
Main Authors: Pollock, Steve V., Mukherjee, Bratati, Bajsa-Hirschel, Joanna, Machingura, Marylou C., Mukherjee, Ananya, Grossman, Arthur R., Moroney, James V.
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2017
Assuntos:
Methodology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5376698/
https://ncbi.nlm.nih.gov/pubmed/28392829
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13007-017-0170-x
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