Reverse genetics in Chlamydomonas: a platform for isolating insertional mutants

A method was developed to identify insertional mutants of Chlamydomonas reinhardtii disrupted for selected target genes. The approach relies on the generation of thousands of transformants followed by PCR-based screenings that allow for identification of strains harboring the introduced marker gene...

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Autors principals: Gonzalez-Ballester, David, Pootakham, Wirulda, Mus, Florence, Yang, Wenqiang, Catalanotti, Claudia, Magneschi, Leonardo, de Montaigu, Amaury, Higuera, Jose J, Prior, Matthew, Galván, Aurora, Fernandez, Emilio, Grossman, Arthur R
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2011
Matèries:
Methodology
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3161022/
https://ncbi.nlm.nih.gov/pubmed/21794168
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1746-4811-7-24
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