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A robust protocol for efficient generation, and genomic characterization of insertional mutants of Chlamydomonas reinhardtii

BACKGROUND: Random insertional mutagenesis of Chlamydomonas reinhardtii using drug resistance cassettes has contributed to the generation of tens of thousands of transformants in dozens of labs around the world. In many instances these insertional mutants have helped elucidate the genetic basis of v...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Plant Methods
Prif Awduron: Pollock, Steve V., Mukherjee, Bratati, Bajsa-Hirschel, Joanna, Machingura, Marylou C., Mukherjee, Ananya, Grossman, Arthur R., Moroney, James V.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: BioMed Central 2017
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC5376698/
https://ncbi.nlm.nih.gov/pubmed/28392829
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13007-017-0170-x
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