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Determination of histone H2AX phosphorylation in DT40 cells

Visualization of DNA damage response protein recruitment to DNA damage sites enables measurement of the DNA damage. DNA double-strand breaks (DSBs) and blocked replication forks induce the phosphorylation of H2AX at serine 139 (γH2AX), and accumulate γH2AX which can then be detected as foci. The det...

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Sonraí Bibleagrafaíochta
Foilsithe in:Methods Mol Biol
Main Authors: Nishihara, Kana, Shahane, Sampada A., Xia, Menghang
Formáid: Artigo
Teanga:Inglês
Foilsithe: 2016
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5375169/
https://ncbi.nlm.nih.gov/pubmed/27518625
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-6346-1_8
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