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Determination of histone H2AX phosphorylation in DT40 cells
Visualization of DNA damage response protein recruitment to DNA damage sites enables measurement of the DNA damage. DNA double-strand breaks (DSBs) and blocked replication forks induce the phosphorylation of H2AX at serine 139 (γH2AX), and accumulate γH2AX which can then be detected as foci. The det...
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| Publié dans: | Methods Mol Biol |
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| Auteurs principaux: | , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2016
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5375169/ https://ncbi.nlm.nih.gov/pubmed/27518625 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-6346-1_8 |
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