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Fluorogenic protein labeling using a genetically encoded unstrained alkene

We developed a new fluorogenic bioorthogonal reaction that is based on the inverse electron-demand Diels–Alder reaction between styrene (an unstrained alkene) and a simple tetrazine. The reaction forms a new fluorophore with no literature precedent. We have identified an aminoacyl-tRNA synthetase/tR...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Chem Sci
Prif Awduron: Shang, X., Song, X., Faller, C., Lai, R., Li, H., Cerny, R., Niu, W., Guo, J.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Royal Society of Chemistry 2017
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC5369545/
https://ncbi.nlm.nih.gov/pubmed/28451254
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c6sc03635j
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