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Fluorogenic protein labeling using a genetically encoded unstrained alkene
We developed a new fluorogenic bioorthogonal reaction that is based on the inverse electron-demand Diels–Alder reaction between styrene (an unstrained alkene) and a simple tetrazine. The reaction forms a new fluorophore with no literature precedent. We have identified an aminoacyl-tRNA synthetase/tR...
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| Veröffentlicht in: | Chem Sci |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Royal Society of Chemistry
2017
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5369545/ https://ncbi.nlm.nih.gov/pubmed/28451254 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c6sc03635j |
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