Cloning, expression, and purification of human cyclophilin in Escherichia coli and assessment of the catalytic role of cysteines by site-directed mutagenesis.

The cDNA encoding human cyclophilin from the Jurkat T-cell lymphoma line has been cloned by the expression cassette polymerase chain reaction and sequenced, and an expression vector has been constructed under control of the tac promoter for efficient expression in Escherichia coli. Active cyclophili...

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Yayımlandı:	Proc Natl Acad Sci U S A
Asıl Yazarlar:	Liu, J, Albers, M W, Chen, C M, Schreiber, S L, Walsh, C T
Materyal Türü:	Artigo
Dil:	Inglês
Baskı/Yayın Bilgisi:	National Academy of Sciences 1990
Konular:	Research Article
Online Erişim:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC53675/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2179953/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.6.2304
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