Cloning, expression, and purification of human cyclophilin in Escherichia coli and assessment of the catalytic role of cysteines by site-directed mutagenesis.

The cDNA encoding human cyclophilin from the Jurkat T-cell lymphoma line has been cloned by the expression cassette polymerase chain reaction and sequenced, and an expression vector has been constructed under control of the tac promoter for efficient expression in Escherichia coli. Active cyclophili...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Liu, J, Albers, M W, Chen, C M, Schreiber, S L, Walsh, C T
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 1990
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC53675/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2179953/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.6.2304
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