Cloning, expression, and purification of human cyclophilin in Escherichia coli and assessment of the catalytic role of cysteines by site-directed mutagenesis.

The cDNA encoding human cyclophilin from the Jurkat T-cell lymphoma line has been cloned by the expression cassette polymerase chain reaction and sequenced, and an expression vector has been constructed under control of the tac promoter for efficient expression in Escherichia coli. Active cyclophili...

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izdano v:	Proc Natl Acad Sci U S A
Principais autores:	Liu, J, Albers, M W, Chen, C M, Schreiber, S L, Walsh, C T
Format:	Artigo
Jezik:	Inglês
Izdano:	National Academy of Sciences 1990
Teme:	Research Article
Online dostop:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC53675/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2179953/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.6.2304
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