Overproduction and dissection of proteins by the expression-cassette polymerase chain reaction.

We report an efficient, general approach for the construction of protein-overproducing strains of Escherichia coli. The method, expression-cassette polymerase chain reaction (ECPCR), allows the insertion of virtually any contiguous coding sequence between sequences that direct high-level protein bio...

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Bibliographic Details
Main Authors: MacFerrin, K D, Terranova, M P, Schreiber, S L, Verdine, G L
Format: Artigo
Language:Inglês
Published: 1990
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC53599/
https://ncbi.nlm.nih.gov/pubmed/2408046
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