Overproduction and dissection of proteins by the expression-cassette polymerase chain reaction.
We report an efficient, general approach for the construction of protein-overproducing strains of Escherichia coli. The method, expression-cassette polymerase chain reaction (ECPCR), allows the insertion of virtually any contiguous coding sequence between sequences that direct high-level protein bio...
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| Udgivet i: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
1990
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC53599/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2408046/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.5.1937 |
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