Overproduction and dissection of proteins by the expression-cassette polymerase chain reaction.

We report an efficient, general approach for the construction of protein-overproducing strains of Escherichia coli. The method, expression-cassette polymerase chain reaction (ECPCR), allows the insertion of virtually any contiguous coding sequence between sequences that direct high-level protein bio...

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Publié dans:	Proc Natl Acad Sci U S A
Auteurs principaux:	MacFerrin, K D, Terranova, M P, Schreiber, S L, Verdine, G L
Format:	Artigo
Langue:	Inglês
Publié:	National Academy of Sciences 1990
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC53599/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2408046/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.5.1937
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