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Efficient, footprint-free human iPSC genome editing by consolidation of Cas9/CRISPR and piggyBac technologies
Genome editing of human induced pluripotent stem cells (iPSCs) offers unprecedented opportunities for in vitro disease modeling and personalized cell replacement therapy. The introduction of Cas9-directed genome editing has expanded adoption of this approach. However, marker free genome editing usin...
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Publicado no: | Nat Protoc |
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Main Authors: | , , , , , , , , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
2016
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5352979/ https://ncbi.nlm.nih.gov/pubmed/27929521 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2016.152 |
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