Efficient, footprint-free human iPSC genome editing by consolidation of Cas9/CRISPR and piggyBac technologies

Genome editing of human induced pluripotent stem cells (iPSCs) offers unprecedented opportunities for in vitro disease modeling and personalized cell replacement therapy. The introduction of Cas9-directed genome editing has expanded adoption of this approach. However, marker free genome editing usin...

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Pubblicato in:Nat Protoc
Autori principali: Wang, Gang, Yang, Luhan, Grishin, Dennis, Ye, Lillian Y., Hu, Yong, Li, Kai, Zhang, Donghui, Villanueva, Xavier R., Church, George M., Pu, William T.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2016
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5352979/
https://ncbi.nlm.nih.gov/pubmed/27929521
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2016.152
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