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Restoration of Gene Function by Homologous Recombination: from PCR to Gene Expression in One Step
We have developed a simple method for single-step cloning of any PCR product into a plasmid. A novel selection principle has been applied, in which activation of a drug selection marker is achieved following homologous recombination. In this method a DNA fragment is amplified by PCR with standard ol...
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| Опубликовано в: : | Appl Environ Microbiol |
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| Главные авторы: | , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
American Society for Microbiology (ASM)
2004
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC535207/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15574912/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.70.12.7156-7160.2004 |
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