Llwytho...
Restoration of Gene Function by Homologous Recombination: from PCR to Gene Expression in One Step
We have developed a simple method for single-step cloning of any PCR product into a plasmid. A novel selection principle has been applied, in which activation of a drug selection marker is achieved following homologous recombination. In this method a DNA fragment is amplified by PCR with standard ol...
Wedi'i Gadw mewn:
| Prif Awduron: | , , , |
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| Fformat: | Artigo |
| Iaith: | Inglês |
| Cyhoeddwyd: |
American Society for Microbiology
2004
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| Pynciau: | |
| Mynediad Ar-lein: | https://ncbi.nlm.nih.gov/pmc/articles/PMC535207/ https://ncbi.nlm.nih.gov/pubmed/15574912 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.12.7156-7160.2004 |
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