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Type II restriction endonuclease R.KpnI is a member of the HNH nuclease superfamily
The restriction endonuclease (REase) R.KpnI is an orthodox Type IIP enzyme, which binds to DNA in the absence of metal ions and cleaves the DNA sequence 5′-GGTAC^C-3′ in the presence of Mg(2+) as shown generating 3′ four base overhangs. Bioinformatics analysis reveals that R.KpnI contains a ββα-Me-f...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC534630/ https://ncbi.nlm.nih.gov/pubmed/15562004 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkh951 |
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