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RNA polymerase III initiation on coligo DNA templates containing loops of variable sequence, size and nucleotide chemistry

Circularized oligonucleotides, or coligos, were previously found to serve as RNA polymerase III (Pol III) templates in vitro and in human tissue culture cells. Here we randomized the 12-nucleotide larger loop (L-loop) of a well characterized coligo and found unexpectedly that in vitro transcription...

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Detalhes bibliográficos
Publicado no:Gene
Main Authors: Patel, Joy, Lama, Lodoe, Hoffmann, Niklas A., Cobo, Jose, Müller, Christoph W., Ryan, Kevin
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5334452/
https://ncbi.nlm.nih.gov/pubmed/27593562
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.gene.2016.09.001
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