Scalable Design of Paired CRISPR Guide RNAs for Genomic Deletion

CRISPR-Cas9 technology can be used to engineer precise genomic deletions with pairs of single guide RNAs (sgRNAs). This approach has been widely adopted for diverse applications, from disease modelling of individual loci, to parallelized loss-of-function screens of thousands of regulatory elements....

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:PLoS Comput Biol
Prif Awduron: Pulido-Quetglas, Carlos, Aparicio-Prat, Estel, Arnan, Carme, Polidori, Taisia, Hermoso, Toni, Palumbo, Emilio, Ponomarenko, Julia, Guigo, Roderic, Johnson, Rory
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Public Library of Science 2017
Pynciau:
Research Article
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC5333799/
https://ncbi.nlm.nih.gov/pubmed/28253259
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pcbi.1005341
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