Scalable Design of Paired CRISPR Guide RNAs for Genomic Deletion

CRISPR-Cas9 technology can be used to engineer precise genomic deletions with pairs of single guide RNAs (sgRNAs). This approach has been widely adopted for diverse applications, from disease modelling of individual loci, to parallelized loss-of-function screens of thousands of regulatory elements....

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Veröffentlicht in:PLoS Comput Biol
Hauptverfasser: Pulido-Quetglas, Carlos, Aparicio-Prat, Estel, Arnan, Carme, Polidori, Taisia, Hermoso, Toni, Palumbo, Emilio, Ponomarenko, Julia, Guigo, Roderic, Johnson, Rory
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2017
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5333799/
https://ncbi.nlm.nih.gov/pubmed/28253259
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pcbi.1005341
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