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Scalable Design of Paired CRISPR Guide RNAs for Genomic Deletion
CRISPR-Cas9 technology can be used to engineer precise genomic deletions with pairs of single guide RNAs (sgRNAs). This approach has been widely adopted for diverse applications, from disease modelling of individual loci, to parallelized loss-of-function screens of thousands of regulatory elements....
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| Publicado no: | PLoS Comput Biol |
|---|---|
| Main Authors: | , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5333799/ https://ncbi.nlm.nih.gov/pubmed/28253259 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pcbi.1005341 |
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