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Glyco-seek: Ultrasensitive Detection of Protein-Specific Glycosylation by Proximity Ligation Polymerase Chain Reaction

We report a non-destructive biochemical technique, termed “Glyco-seek”, for analysis of O-GlcNAcylated proteins. Glyco-seek combines chemoenzymatic labeling, proximity ligation, and quantitative polymerase chain reaction to detect O-GlcNAcylated proteins with ultrahigh sensitivity. Our glycan-specif...

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Bibliographic Details
Published in:J Am Chem Soc
Main Authors: Robinson, Peter V., Tsai, Cheng-ting, de Groot, Amber E., McKechnie, Julia L., Bertozzi, Carolyn R.
Format: Artigo
Language:Inglês
Published: 2016
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5327792/
https://ncbi.nlm.nih.gov/pubmed/27454154
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jacs.6b03861
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