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Glyco-seek: Ultrasensitive Detection of Protein-Specific Glycosylation by Proximity Ligation Polymerase Chain Reaction
We report a non-destructive biochemical technique, termed “Glyco-seek”, for analysis of O-GlcNAcylated proteins. Glyco-seek combines chemoenzymatic labeling, proximity ligation, and quantitative polymerase chain reaction to detect O-GlcNAcylated proteins with ultrahigh sensitivity. Our glycan-specif...
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| Publicado no: | J Am Chem Soc |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5327792/ https://ncbi.nlm.nih.gov/pubmed/27454154 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jacs.6b03861 |
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