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Active yeast ribosome preparation using monolithic anion exchange chromatography
In vitro studies of translation provide critical mechanistic details, yet purification of large amounts of highly active eukaryotic ribosomes remains a challenge for biochemists and structural biologists. Here, we present an optimized method for preparation of highly active yeast ribosomes that coul...
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| Publicat a: | RNA Biol |
|---|---|
| Autors principals: | , , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Taylor & Francis
2016
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5324736/ https://ncbi.nlm.nih.gov/pubmed/27981882 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/15476286.2016.1270004 |
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