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Active yeast ribosome preparation using monolithic anion exchange chromatography

In vitro studies of translation provide critical mechanistic details, yet purification of large amounts of highly active eukaryotic ribosomes remains a challenge for biochemists and structural biologists. Here, we present an optimized method for preparation of highly active yeast ribosomes that coul...

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Detalhes bibliográficos
Publicado no:RNA Biol
Main Authors: Munoz, Antonio M., Yourik, Paul, Rajagopal, Vaishnavi, Nanda, Jagpreet S., Lorsch, Jon R., Walker, Sarah E.
Formato: Artigo
Idioma:Inglês
Publicado em: Taylor & Francis 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5324736/
https://ncbi.nlm.nih.gov/pubmed/27981882
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/15476286.2016.1270004
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