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Isolation of Bacterial Ribosomes with Monolith Chromatography

We report the development of a rapid chromatographic method for the isolation of bacterial ribosomes from crude cell lysates in less than ten minutes. Our separation is based on the use of strong anion exchange monolithic columns. Using a simple stepwise elution program we were able to purify riboso...

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Detalhes bibliográficos
Main Authors: Trauner, Andrej, Bennett, Mark H., Williams, Huw D.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3033897/
https://ncbi.nlm.nih.gov/pubmed/21326610
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0016273
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