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Isolation of Bacterial Ribosomes with Monolith Chromatography

We report the development of a rapid chromatographic method for the isolation of bacterial ribosomes from crude cell lysates in less than ten minutes. Our separation is based on the use of strong anion exchange monolithic columns. Using a simple stepwise elution program we were able to purify riboso...

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Autors principals: Trauner, Andrej, Bennett, Mark H., Williams, Huw D.
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2011
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3033897/
https://ncbi.nlm.nih.gov/pubmed/21326610
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0016273
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