Highly sensitive and adaptable fluorescence-quenched pair discloses the substrate specificity profiles in diverse protease families

Internally quenched fluorescent (IQF) peptide substrates originating from FRET (Förster Resonance Energy Transfer) are powerful tool for examining the activity and specificity of proteases, and a variety of donor/acceptor pairs are extensively used to design individual substrates and combinatorial l...

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Gepubliceerd in:Sci Rep
Hoofdauteurs: Poreba, Marcin, Szalek, Aleksandra, Rut, Wioletta, Kasperkiewicz, Paulina, Rutkowska-Wlodarczyk, Izabela, Snipas, Scott J., Itoh, Yoshifumi, Turk, Dusan, Turk, Boris, Overall, Christopher M., Kaczmarek, Leszek, Salvesen, Guy S., Drag, Marcin
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Nature Publishing Group 2017
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Article
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5322338/
https://ncbi.nlm.nih.gov/pubmed/28230157
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep43135
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