Biochemical Characterization of the THIN-B Metallo-β-Lactamase of Janthinobacterium lividum

The THIN-B metallo-β-lactamase, a subclass B3 enzyme produced by the environmental species Janthinobacterium lividum, was overproduced in Escherichia coli by means of a T7-based expression system. The enzyme was purified (>95%) by two ion-exchange chromatography steps and subjected to biochemical...

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Bibliographic Details
Main Authors: Docquier, Jean-Denis, Lopizzo, Teresa, Liberatori, Sabrina, Prenna, Manuela, Thaller, Maria Cristina, Frère, Jean-Marie, Rossolini, Gian Maria
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2004
Subjects:
Mechanisms of Resistance
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC529184/
https://ncbi.nlm.nih.gov/pubmed/15561856
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.48.12.4778-4783.2004
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