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Biochemical Characterization of the THIN-B Metallo-β-Lactamase of Janthinobacterium lividum
The THIN-B metallo-β-lactamase, a subclass B3 enzyme produced by the environmental species Janthinobacterium lividum, was overproduced in Escherichia coli by means of a T7-based expression system. The enzyme was purified (>95%) by two ion-exchange chromatography steps and subjected to biochemical...
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| Main Authors: | , , , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology
2004
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC529184/ https://ncbi.nlm.nih.gov/pubmed/15561856 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.48.12.4778-4783.2004 |
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