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Purification and Biochemical Characterization of IMP-13 Metallo-β-Lactamase
The IMP-13 metallo-β-lactamase was overproduced in Escherichia coli BL21(DE3) and purified by chromatography. Analysis of kinetic parameters revealed some notable differences with other IMP-type enzymes, noteworthily a higher catalytic efficiency toward ticarcillin and piperacillin and a marked pref...
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| Autores principales: | , , , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
American Society for Microbiology (ASM)
2011
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3019620/ https://ncbi.nlm.nih.gov/pubmed/20974864 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.00421-10 |
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