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Purification and Biochemical Characterization of IMP-13 Metallo-β-Lactamase

The IMP-13 metallo-β-lactamase was overproduced in Escherichia coli BL21(DE3) and purified by chromatography. Analysis of kinetic parameters revealed some notable differences with other IMP-type enzymes, noteworthily a higher catalytic efficiency toward ticarcillin and piperacillin and a marked pref...

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Main Authors: Santella, Gisela, Docquier, Jean-Denis, Gutkind, Gabriel, Rossolini, Gian Maria, Radice, Marcela
格式: Artigo
語言:Inglês
出版: American Society for Microbiology (ASM) 2011
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3019620/
https://ncbi.nlm.nih.gov/pubmed/20974864
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.00421-10
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