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Design of Trypanosoma rangeli sialidase mutants with improved trans-sialidase activity

A sialidase (EC 3.2.1.18) from the non-pathogenic Trypanosoma rangeli, TrSA, has been shown to exert trans-sialidase activity after mutation of five specific amino acids in the active site (M96V, A98P, S120Y, G249Y, Q284P) to form the so-called TrSA(5mut) enzyme. By computational and hypothesis driv...

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Vydáno v:PLoS One
Hlavní autoři: Nyffenegger, Christian, Nordvang, Rune Thorbjørn, Jers, Carsten, Meyer, Anne S., Mikkelsen, Jørn Dalgaard
Médium: Artigo
Jazyk:Inglês
Vydáno: Public Library of Science 2017
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5291517/
https://ncbi.nlm.nih.gov/pubmed/28158299
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0171585
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