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Proximity-dependent biotin labeling in yeast using the engineered ascorbate peroxidase APEX2
The engineered ascorbate peroxidase (APEX2) has been effectively employed in mammalian cells to identify protein-protein interactions. APEX2 fused to a protein of interest covalently tags nearby proteins with biotin-phenol (BP) when H(2)O(2) is added to the cell culture medium. Subsequent affinity p...
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| Publicado no: | Biochem J |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5290329/ https://ncbi.nlm.nih.gov/pubmed/27274088 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BCJ20160106 |
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