Mapping protein networks in yeast mitochondria using proximity-dependent biotin identification coupled to proteomics

Proximity-dependent biotin identification (BioID) permits biotinylation of proteins interacting directly, indirectly, or just localized in proximity of a protein of interest (bait). Here, we describe how BioID coupled to proteomics and network biology can be used to map protein proximities in yeast...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:STAR Protoc
Main Authors: Salvatori, Roger, Aftab, Wasim, Forne, Ignasi, Imhof, Axel, Ott, Martin, Singh, Abeer Prakash
Format: Artigo
Jezik:Inglês
Izdano: Elsevier 2020
Teme:
Protocol
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7757666/
https://ncbi.nlm.nih.gov/pubmed/33377112
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100219
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