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Exploring the energy landscape of GFP by single-molecule mechanical experiments
We use single-molecule force spectroscopy to drive single GFP molecules from the native state through their complex energy landscape into the completely unfolded state. Unlike many smaller proteins, mechanical GFP unfolding proceeds by means of two subsequent intermediate states. The transition from...
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| Hauptverfasser: | , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
National Academy of Sciences
2004
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC528946/ https://ncbi.nlm.nih.gov/pubmed/15531635 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0404549101 |
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