Concerted Assembly and Cloning of Multiple DNA Segments Using In Vitro Site-Specific Recombination: Functional Analysis of Multi-Segment Expression Clones

The ability to clone and manipulate DNA segments is central to molecular methods that enable expression, screening, and functional characterization of genes, proteins, and regulatory elements. We previously described the development of a novel technology that utilizes in vitro site-specific recombin...

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Autors principals: Cheo, David L., Titus, Steven A., Byrd, Devon R.N., Hartley, James L., Temple, Gary F., Brasch, Michael A.
Format: Artigo
Idioma:Inglês
Publicat: Cold Spring Harbor Laboratory Press 2004
Matèries:
Methods
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC528927/
https://ncbi.nlm.nih.gov/pubmed/15489333
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.2512204
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