Concerted Assembly and Cloning of Multiple DNA Segments Using In Vitro Site-Specific Recombination: Functional Analysis of Multi-Segment Expression Clones

The ability to clone and manipulate DNA segments is central to molecular methods that enable expression, screening, and functional characterization of genes, proteins, and regulatory elements. We previously described the development of a novel technology that utilizes in vitro site-specific recombin...

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Main Authors: Cheo, David L., Titus, Steven A., Byrd, Devon R.N., Hartley, James L., Temple, Gary F., Brasch, Michael A.
格式: Artigo
語言:Inglês
出版: Cold Spring Harbor Laboratory Press 2004
主題:
Methods
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC528927/
https://ncbi.nlm.nih.gov/pubmed/15489333
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.2512204
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