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Feasibility of Genome-Scale Construction of Promoter::Reporter Gene Fusions for Expression in Caenorhabditis elegans Using a MultiSite Gateway Recombination System

The understanding of gene function increasingly requires the characterization of DNA segments containing promoters and their associated regulatory sequences. We describe a novel approach for linking multiple DNA segments, here applied to the generation of promoter::reporter fusions. Promoters from C...

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Detalhes bibliográficos
Main Authors: Hope, Ian A., Stevens, Jonathan, Garner, Anna, Hayes, Josie, Cheo, David L., Brasch, Michael A., Vidal, Marc
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2004
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC528922/
https://ncbi.nlm.nih.gov/pubmed/15489328
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.2463804
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