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Enhancing yields of low and single copy number plasmid DNAs from E. coli cells

Many plasmids used for gene cloning and heterologous protein expression in E. coli cells are low copy number or single copy number plasmids. Extraction of these types of plasmids from small bacterial cell cultures produces low DNA yields. In this study we have quantitated yields of low copy and sing...

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Detaylı Bibliyografya
Yayımlandı:J Microbiol Methods
Asıl Yazarlar: Wood, Whitney N., Smith, Kyle D., Ream, Jennifer A., Lewis, L. Kevin
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2016
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC5286560/
https://ncbi.nlm.nih.gov/pubmed/28024984
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mimet.2016.12.016
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