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Enhancing yields of low and single copy number plasmid DNAs from E. coli cells
Many plasmids used for gene cloning and heterologous protein expression in E. coli cells are low copy number or single copy number plasmids. Extraction of these types of plasmids from small bacterial cell cultures produces low DNA yields. In this study we have quantitated yields of low copy and sing...
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Publicado no: | J Microbiol Methods |
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Main Authors: | , , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
2016
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5286560/ https://ncbi.nlm.nih.gov/pubmed/28024984 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mimet.2016.12.016 |
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