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Using the Volta phase plate with defocus for cryo-EM single particle analysis

Previously, we reported an in-focus data acquisition method for cryo-EM single-particle analysis with the Volta phase plate (Danev and Baumeister, 2016). Here, we extend the technique to include a small amount of defocus which enables contrast transfer function measurement and correction. This hybri...

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Detalhes bibliográficos
Publicado no:eLife
Main Authors: Danev, Radostin, Tegunov, Dimitry, Baumeister, Wolfgang
Formato: Artigo
Idioma:Inglês
Publicado em: eLife Sciences Publications, Ltd 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5279940/
https://ncbi.nlm.nih.gov/pubmed/28109158
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.23006
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