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Using the Volta phase plate with defocus for cryo-EM single particle analysis
Previously, we reported an in-focus data acquisition method for cryo-EM single-particle analysis with the Volta phase plate (Danev and Baumeister, 2016). Here, we extend the technique to include a small amount of defocus which enables contrast transfer function measurement and correction. This hybri...
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| Veröffentlicht in: | eLife |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
eLife Sciences Publications, Ltd
2017
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5279940/ https://ncbi.nlm.nih.gov/pubmed/28109158 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.23006 |
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