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Cryo-EM single particle analysis with the Volta phase plate
We present a method for in-focus data acquisition with a phase plate that enables near-atomic resolution single particle reconstructions. Accurate focusing is the determining factor for obtaining high quality data. A double-area focusing strategy was implemented in order to achieve the required prec...
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| 發表在: | eLife |
|---|---|
| Main Authors: | , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
eLife Sciences Publications, Ltd
2016
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4850076/ https://ncbi.nlm.nih.gov/pubmed/26949259 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.13046 |
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