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Cryo-EM single particle analysis with the Volta phase plate

We present a method for in-focus data acquisition with a phase plate that enables near-atomic resolution single particle reconstructions. Accurate focusing is the determining factor for obtaining high quality data. A double-area focusing strategy was implemented in order to achieve the required prec...

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Vydáno v:eLife
Hlavní autoři: Danev, Radostin, Baumeister, Wolfgang
Médium: Artigo
Jazyk:Inglês
Vydáno: eLife Sciences Publications, Ltd 2016
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4850076/
https://ncbi.nlm.nih.gov/pubmed/26949259
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.13046
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